An improved fixation method for chromosome preparation of Chinese hamster, Chinese hamster-human hybrid, and mouse cell lines

Cytogenet Cell Genet. 1986;41(3):181-4. doi: 10.1159/000132224.


Lowering the proportion of acetic acid in the standard 1:3 acetic acid:methanol chromosome fixative used both during initial fixation and subsequent washing produced up to a 20-fold increase in the yield of intact metaphases from cultures of several permanent cell lines. Although this inhibited chromosome spreading, addition of various acetic acid-methanol mixtures immediately after the cell suspension was dropped onto slides increased the degree of spreading and resulted in well-spread, cytoplasm-free metaphases.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cell Line
  • Cells, Cultured
  • Chromosome Banding
  • Chromosomes / ultrastructure*
  • Chromosomes, Human / ultrastructure*
  • Cricetinae
  • Cricetulus
  • Humans
  • Hybrid Cells / cytology*
  • Karyotyping
  • Lymphocytes / cytology*
  • Mice
  • Species Specificity