Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining

Exp Cell Res. 1986 Apr;163(2):309-16. doi: 10.1016/0014-4827(86)90062-5.

Abstract

On staining with a monoclonal antibody raised against microtubule-associated protein-1 (MAP-1), dot-like structures were seen in the nuclei of interphase cells, but not in those of non-cycling G0-arrested cells. Dots were also not seen in the nuclei of non-cycling senescent human cells (IMR-90). A SV40-DNA-transformed subline of IMR-90 with a limited growth potential showed progressive decrease of cells with nuclei containing dots in the final stage of their lifespan. The dots appeared in G0-arrested IMR-90 cells when these cells were incubated in medium of high osmotic pressure for 3 min. In contrast, no dots appeared in senescent cells or X-ray-irradiated young cells when they were incubated in medium of high osmotic pressure. Thus irreversibly non-cycling cells could be distinguished from G0-phase cells on the level of whole cultures. The results suggest that senescent cells lose their division potential by entering an irreversible cell-cycle stage differing from G0.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Cell Cycle
  • Cell Line
  • Cell Nucleus / ultrastructure
  • Culture Media
  • Female
  • Fibroblasts / cytology
  • Humans
  • Interphase
  • Kinetics
  • Lung / cytology*
  • Lung / embryology
  • Microtubule-Associated Proteins / analysis*
  • Osmolar Concentration
  • Pregnancy

Substances

  • Antibodies, Monoclonal
  • Culture Media
  • Microtubule-Associated Proteins