Phosphorylation of 3-O-methyl-D-glucose by yeast and beef hexokinase

FEBS Lett. 1986 Mar 31;198(2):292-4. doi: 10.1016/0014-5793(86)80423-9.

Abstract

Beef heart hexokinase and yeast hexokinase both catalyzed the phosphorylation of 3-O-[14C]methyl-D-glucose. The maximal velocity was 3 orders of magnitude lower and the Km for the glucose analogue 40-120-times higher than those observed with D-[U-14C]glucose. Hence, 3-O-methyl-D-glucose should not be considered as a truly nonmetabolized analogue of D-glucose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3-O-Methylglucose
  • Animals
  • Carbon Radioisotopes
  • Cattle
  • Glucose / metabolism
  • Hexokinase / pharmacology*
  • Kinetics
  • Methylglucosides / metabolism*
  • Methylglycosides / metabolism*
  • Phosphorylation
  • Yeasts / enzymology

Substances

  • Carbon Radioisotopes
  • Methylglucosides
  • Methylglycosides
  • 3-O-Methylglucose
  • Hexokinase
  • Glucose