An analysis of class I antigens of man and other species by one-dimensional IEF and immunoblotting

Immunogenetics. 1986;23(3):164-71. doi: 10.1007/BF00373817.

Abstract

A procedure for the molecular identification of MHC class I products based on 1-D IEF and subsequent immunoblotting is described. Optimal conditions for 1-D IEF, the electrophoretic transfer of proteins out of denaturing, nonionic detergent-containing gels to nitrocellulose, and the requisite antibodies, both polyclonal and monoclonal, for the visualization of class I heavy chains have been established. Cross-reactivity of antibodies has enabled the biochemical analysis of class I heavy chains in the dog. The procedure reported here requires modest amounts of cells and allows a rapid molecular characterization of class I heavy chain polymorphisms in man and other species without the need for radiochemical methods.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cats / immunology
  • Dogs / immunology
  • HLA Antigens / analysis*
  • Histocompatibility Antigens / analysis*
  • Humans
  • Immunoelectrophoresis
  • Isoelectric Focusing
  • Major Histocompatibility Complex
  • Mice / immunology
  • Species Specificity

Substances

  • HLA Antigens
  • Histocompatibility Antigens