Orthopoxvirus Purification for High Containment Laboratories

Laura Hughes; Kimberly Wilkins; Scott Smith; Victoria A Olson; Panayampalli Subbian Satheshkumar

doi:10.1007/978-1-0716-4160-6_10

Orthopoxvirus Purification for High Containment Laboratories

Methods Mol Biol. 2025:2860:149-156. doi: 10.1007/978-1-0716-4160-6_10.

Authors

Laura Hughes¹, Kimberly Wilkins², Scott Smith², Victoria A Olson², Panayampalli Subbian Satheshkumar²

Affiliations

¹ Poxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA. bkz2@cdc.gov.
² Poxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.

PMID: 39621266
DOI: 10.1007/978-1-0716-4160-6_10

Abstract

Purification of large viruses in a high containment laboratory can create unique challenges. Traditional purification methods for large viruses rely on equipment and techniques that are not ideal for high containment work. Poxvirus purification has long relied on the use of Genetron^®, a reagent that is no longer available. Here we describe a purification protocol that is effective for semi-purification of orthopoxviruses and suitable for work in high containment laboratories.

Keywords: High containment laboratory; Orthopoxvirus; Poxvirus; Virus purification.

MeSH terms

Animals
Containment of Biohazards / methods
Laboratories
Orthopoxvirus* / isolation & purification