Purification of small oligonucleotides by polyacrylamide gel electrophoresis and transfer to diethylaminoethyl paper

Anal Biochem. 1986 Feb 1;152(2):221-5. doi: 10.1016/0003-2697(86)90401-x.

Abstract

The application of polyacrylamide gel electrophoresis with subsequent electroelution onto DEAE paper for the purification of small oligonucleotides is described. We demonstrate that synthetic DNAs and hydrolyzed RNAs as small as three nucleotides in length can be purified by this technique. The product is undegraded and homogeneous in length.

MeSH terms

  • Electrophoresis, Polyacrylamide Gel / methods*
  • Ethanolamines
  • Oligodeoxyribonucleotides / isolation & purification
  • Oligonucleotides / isolation & purification*
  • Oligoribonucleotides / isolation & purification
  • Paper

Substances

  • Ethanolamines
  • Oligodeoxyribonucleotides
  • Oligonucleotides
  • Oligoribonucleotides
  • 2-diethylaminoethanol