ADP-ribosylation induced changes in the conformation of the chromatin of the brain of developing rats

Biochem Int. 1986 Feb;12(2):303-11.

Abstract

Conformational changes in the chromatin of the cerebral hemisphere of 3-, 14- and 30-day old developing rats were studied before and after its ADP-ribosylation using DNase I and micrococcal nuclease (MNase). The rate and extent of digestion of chromatin by DNase I are the highest at 3-day and decline progressively thereafter. The rate and extent of digestion by MNase do not change during development. ADP-ribosylation of chromosomal proteins was carried out by incubating nuclei with NAD+ for 30 min and was followed by endonuclease digestion. Both the rate and extent of digestion by DNase I and MNase were enhanced after ADP-ribosylation which was the maximum for 3-day rats.

MeSH terms

  • Adenosine Diphosphate Ribose / pharmacology*
  • Aging
  • Animals
  • Brain / growth & development*
  • Cell Nucleus / ultrastructure
  • Chromatin / drug effects
  • Chromatin / ultrastructure*
  • DNA / isolation & purification
  • Deoxyribonuclease I / metabolism
  • Kinetics
  • Male
  • Micrococcal Nuclease / metabolism
  • Nucleoside Diphosphate Sugars / pharmacology*
  • Rats
  • Rats, Inbred Strains

Substances

  • Chromatin
  • Nucleoside Diphosphate Sugars
  • Adenosine Diphosphate Ribose
  • DNA
  • Deoxyribonuclease I
  • Micrococcal Nuclease