Generation and characterization of the LINC01405 knockout human embryonic stem cell line

Yaping Xu; Hongchun Wu; Jingxiu Jiang; Lingqun Ye; Kaili Hao; Kunjun Han; Shijun Hu; Wei Lei; Zhikun Guo

doi:10.1016/j.scr.2024.103619

Generation and characterization of the LINC01405 knockout human embryonic stem cell line

Stem Cell Res. 2024 Dec 1:82:103619. doi: 10.1016/j.scr.2024.103619. Online ahead of print.

Authors

Yaping Xu¹, Hongchun Wu², Jingxiu Jiang², Lingqun Ye², Kaili Hao², Kunjun Han², Shijun Hu², Wei Lei³, Zhikun Guo⁴

Affiliations

¹ The 7th People's Hospital of Zhengzhou, Zhengzhou 450000, Henan, China.
² Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, China.
³ Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, China. Electronic address: leiwei@suda.edu.cn.
⁴ The 7th People's Hospital of Zhengzhou, Zhengzhou 450000, Henan, China. Electronic address: 781003@xxmu.edu.cn.

PMID: 39644600
DOI: 10.1016/j.scr.2024.103619

Abstract

Long Intergenic Non-Protein Coding RNA 1405 (LINC01405), with known elevated expression in muscle, has been linked to a number of musculo-skeletal conditions. By utilizing the CRISPR/Cas9 gene editing system, we generated a LINC01405 knockout human embryonic stem cell (hESC) line. This line remains human stem cell-like morphology and pluripotency, exhibits a normal karyotype, and can differentiate into cells from all three germ layers. This cell line will be an invaluable model for the research on LINC01405's role in normal development of cardiac and skeletal muscle, and their diseases.