Two new species Aeolothrips kermanensis sp. n., from the southeast, and Aeolothrips richardi sp. n. from the west of Iran are described as new to science. Host-plant relationships among Aeolothrips Haliday species of Iran are discussed. The record of A. montivagus from Iran was revised. The list of Iranian species of Aeolothrips is updated and diagnostic characters for the two new species are illustrated.