Intracellular distribution and radiotoxicity of chromium-51 in mammalian cells: Auger-electron dosimetry

J Nucl Med. 1985 Jan;26(1):59-67.


The kinetics of uptake and of radiotoxicity of chromium-51, an Auger-electron emitter, have been studied in V79 lung fibroblasts of the Chinese hamster. Intracellular radioactivity was directly proportional to the incubation period and to the extracellular concentration of the Cr-51. About 14% of the cellular activity was associated with the nucleus, whereas approximately 2% was guanidine-precipitable and therefore bound to DNA. The growth rate of V79 cells was slowed following intracellular incorporation of Cr-51. The cell-survival curve, in terms of colony-forming ability, was of the low-LET type, with a D37 of 6.2 pCi/cell. Theoretical dosimetric estimates indicate that, under the given experimental conditions, the mean lethal dose to the cell nucleus was 870 rad. Although this value is somewhat larger than the x-ray D37 dose of 580 rad for this cell line, it is more realistic than the gross underestimate obtained by classical MIRD calculations (2-3 rad/cell).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Cell Nucleus / radiation effects
  • Cell Survival / radiation effects
  • Chromium Radioisotopes* / adverse effects
  • Cricetinae
  • Cricetulus
  • Electrons
  • Fibroblasts / cytology
  • Fibroblasts / radiation effects
  • Half-Life
  • Kinetics
  • Radiation Dosage*


  • Chromium Radioisotopes