Biochemical properties and kinetic parameters of dihydroxyphenylalanine--5-hydroxytryptophan decarboxylase in brain, liver, and adrenals of cat

Can J Biochem. 1979 Jul;57(7):1014-8. doi: 10.1139/o79-126.

Abstract

Biochemical properties and kinetic parameters of nonpurified dihydroxyphenylalanine-5-hydroxytryptophan decarboxylase extracted from brain and two peripheral organs, liver and adrenals, were studied in the cat. This study shows that decarboxylase activity in brain is lower than in peripheral organs and that 5-hydroxytryptophan can be decarboxylated without exogenous addition of pyridoxal-5'-phosphate (PLP). However, the addition of PLP substantially increases the enzyme activity. Excess of coenzyme (greater than 60 muM) induces inhibition in adrenals and liver but not in the central nervous system (CNS). The observed inhibition might be related to the presence of a tetrahydroisoquinoline derivative formed in the medium. Differentiation between mechanisms of action of decarboxylase in the CNS and peripheral organs is suggested.

Publication types

  • Comparative Study

MeSH terms

  • 5-Hydroxytryptophan
  • Adrenal Glands / enzymology*
  • Animals
  • Aromatic-L-Amino-Acid Decarboxylases / metabolism*
  • Brain / enzymology*
  • Cats
  • Dopa Decarboxylase / metabolism*
  • Female
  • Hydrogen-Ion Concentration
  • Kinetics
  • Liver / enzymology*
  • Male
  • Organ Specificity

Substances

  • 5-Hydroxytryptophan
  • Dopa Decarboxylase
  • Aromatic-L-Amino-Acid Decarboxylases