Phenotypic analysis of temperature-sensitive yeast actin mutants

Cell. 1985 Feb;40(2):405-16. doi: 10.1016/0092-8674(85)90154-0.


The consequences of two different mutations in the single essential actin structural gene of yeast (Saccharomyces cerevisiae) were studied. Both conditional-lethal actin mutants exhibit six phenotypes at the restrictive temperature: disruption of the asymmetric staining pattern of actin assembly; delocalized deposition of chitin on the cell surface; partial inhibition of secretion of the periplasmic protein, invertase; an intracellular accumulation of secretory vesicles; death of cells in the budded portion of the cell cycle upon prolonged incubation at the restrictive condition; and osmotic sensitivity. These results implicate actin in the organization and polarized growth of the yeast cell surface.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / genetics*
  • Cell Cycle
  • Cell Survival
  • Chitin / analysis
  • Chromosomes
  • Glycoside Hydrolases / metabolism
  • Mutation*
  • Osmotic Pressure
  • Phenotype
  • Temperature*
  • Yeasts / genetics*
  • Yeasts / metabolism
  • beta-Fructofuranosidase


  • Actins
  • Chitin
  • Glycoside Hydrolases
  • beta-Fructofuranosidase