Pro-fertility and Antioxidant Potentials of Vigna unguiculata (Cowpea) Protein Isolate and Essential Oil: An In vivo and In silico Studies

Olugbenga Samuel Oladimeji; Olasunkanmi Kayode Awote; Nzubechi Olympian Elum

doi:10.2174/0109298665358634241217094220

Pro-fertility and Antioxidant Potentials of Vigna unguiculata (Cowpea) Protein Isolate and Essential Oil: An In vivo and In silico Studies

Protein Pept Lett. 2025 Jan 3. doi: 10.2174/0109298665358634241217094220. Online ahead of print.

Authors

Olugbenga Samuel Oladimeji¹, Olasunkanmi Kayode Awote¹, Nzubechi Olympian Elum¹

Affiliation

¹ Department of Biochemistry, Faculty of Science, Lagos State University, Ojo, Lagos, Nigeria.

PMID: 39773051
DOI: 10.2174/0109298665358634241217094220

Abstract

Introduction: Vigna unguiculata (Cowpea), a legume rich in phytochemicals, has been traditionally used to improve fertility and treat various ailments. This study used in-silico and in-- vivo methods to evaluate the effects of cowpea protein isolate and essential oil on reproductive hormonal and antioxidant indices.

Method: Forty (40) female rats were divided into eight groups (n=5). After 14 days of treatment, hormone levels (progesterone, prolactin, testosterone, estradiol) and antioxidant activities (superoxide dismutase [SOD], catalase [CAT]) were assessed using biochemical kits and standard procedures. Molecular docking studies were performed using PyRx and Biovia Discovery Studio 2021. The ligands generated from gas chromatography-mass spectroscopy (GCMS) analysis of cowpea oil and the target proteins (SOD and CAT) were from PubChem and RCSB Protein Data Bank, respectively.

Results: The results of this study showed that cowpea oil and protein isolate significantly (p<0.05) reduced plasma CAT and SOD activities while increasing their activities in the ovary and liver tissues compared to the infertile untreated group. Consistent administration of either cowpea oil or protein isolate was observed to positively regulate the hormonal indices in the infertile treated groups. Phthalic acid, 2-cyclohexyl ethyl isobutyl ester demonstrated a strong binding affinity and binding constant with SOD and CAT, which suggests that the ligands from cowpea essential oil have antioxidant and pro-fertility potentials that could be developed to treat fertility-related issues.

Conclusion: Based on the results of this study, it can be concluded that V. unguiculata can promote fertility, possibly through its rich embedded phytochemicals, which substantiates its traditional claim.

Keywords: Vigna unguiculata; antioxidants; essential oil; gas chromatography-mass spectroscopy (GCMS).; infertility; molecular docking; pro-fertility; reproductive hormones.