Protocol for applying Tumor Treating Fields in mouse models of cancer using the inovivo system

Travis J Gates; Sewar Zbidat; Karina Deniz; Sanyukta Padmanabhan; Katherine Ladner; Akshat Sarkari; Xianda Zhao; Shiri Davidi; Roni Blatt; Shay Cahal; Martin Gabay; Mariell Sellevoll; Itai Tzchori; Yaara Porat; Adi Haber; Moshe Giladi; Subbaya Subramanian; Emil Lou

doi:10.1016/j.xpro.2024.103535

Protocol for applying Tumor Treating Fields in mouse models of cancer using the inovivo system

STAR Protoc. 2025 Mar 21;6(1):103535. doi: 10.1016/j.xpro.2024.103535. Epub 2025 Jan 9.

Authors

Travis J Gates¹, Sewar Zbidat², Karina Deniz³, Sanyukta Padmanabhan³, Katherine Ladner³, Akshat Sarkari³, Xianda Zhao⁴, Shiri Davidi², Roni Blatt², Shay Cahal², Martin Gabay², Mariell Sellevoll², Itai Tzchori², Yaara Porat², Adi Haber², Moshe Giladi⁵, Subbaya Subramanian⁶, Emil Lou⁷

Affiliations

¹ Department of Pharmacology, University of Minnesota Medical School, Minneapolis, MN, USA.
² Novocure Ltd., Haifa, Israel.
³ Department of Medicine, Division of Hematology, Oncology, and Transplantation, University of Minnesota Medical School, Minneapolis, MN, USA.
⁴ Department of Surgery, University of Minnesota Medical School, Minneapolis, MN, USA.
⁵ Novocure Ltd., Haifa, Israel. Electronic address: mosheg@novocure.com.
⁶ Department of Surgery, University of Minnesota Medical School, Minneapolis, MN, USA; Masonic Cancer Center, University of Minnesota Medical School, Minneapolis, MN, USA; Center for Immunology, University of Minnesota Medical School, Minneapolis, MN, USA.
⁷ Department of Medicine, Division of Hematology, Oncology, and Transplantation, University of Minnesota Medical School, Minneapolis, MN, USA; Masonic Cancer Center, University of Minnesota Medical School, Minneapolis, MN, USA. Electronic address: emil-lou@umn.edu.

Abstract

Tumor Treating Fields (TTFields) are electric fields clinically approved for cancer treatment, delivered via arrays attached to the patient's skin. Here, we present a protocol for applying TTFields to torso orthotopic and subcutaneous mouse tumor models using the inovivo system. We guide users on proper system component connections, study protocol design, mouse fur depilation, array application, and treatment condition adjustment and monitoring. The inovivo system allows for the concurrent application of TTFields with standard cancer therapies. For complete details on the use and execution of this protocol, please refer to Barsheshet et al.¹.

Keywords: cancer; cell biology; model organisms.

MeSH terms

Animals
Disease Models, Animal
Electric Stimulation Therapy* / instrumentation
Electric Stimulation Therapy* / methods
Mice
Neoplasms* / therapy