DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome

M G Mattei; N Philip; E Passage; J P Moisan; J L Mandel; J F Mattei

doi:10.1007/BF00293038

DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome

Hum Genet. 1985;69(3):268-71. doi: 10.1007/BF00293038.

Authors

M G Mattei, N Philip, E Passage, J P Moisan, J L Mandel, J F Mattei

PMID: 3980018
DOI: 10.1007/BF00293038

Abstract

Recombinant plasmid clone B74 (also named D18 S3) containing a human single-copy DNA segment of 6 kilobases (kb) was localized by in situ hybridization on band p113 of chromosome 18. This probe was then used in cytogenetic diagnosis to identify precisely a small supernumerary chromosome as an isochromosome i(18p).

MeSH terms

Autoradiography
Cells, Cultured
Chromosome Aberrations*
Chromosome Banding
Chromosomes, Human, 16-18*
Cloning, Molecular
DNA / genetics*
Female
Humans
Intellectual Disability / genetics
Karyotyping
Lymphocytes / ultrastructure
Male
Nucleic Acid Hybridization
Trisomy*

Substances

DNA