Eco-friendly extraction and recovery of triterpenoids from persimmon leaves (Diospyros kaki L.f.) using ultrasound-assisted deep eutectic solvents and macroporous resins

Anal Methods. 2025 Feb 13;17(7):1567-1578. doi: 10.1039/d4ay01885k.

Abstract

Triterpenoids are known for their promising biological activities, and there is a growing focus on green extraction methods for these compounds. In this study, ultrasound-assisted deep eutectic solvents were employed to extract triterpenoids from persimmon leaves, with choline chloride-lactic acid identified as an effective green solvent. The extraction conditions were carefully optimized using response surface methodology, resulting in an extraction efficiency of 12.41%, which is 1.54 times higher than that achieved with conventional organic solvents such as methanol. The triterpenoids were then successfully recovered using a solid-liquid extraction method based on AB-8 macroporous resins, achieving a 94.01% recovery rate with a final product triterpenoid content of 81.87%. These findings provide a strong foundation for the sustainable extraction of triterpenoids and other valuable compounds from biomass.

MeSH terms

  • Deep Eutectic Solvents / chemistry
  • Diospyros* / chemistry
  • Green Chemistry Technology / methods
  • Plant Extracts / chemistry
  • Plant Leaves* / chemistry
  • Porosity
  • Triterpenes* / chemistry
  • Triterpenes* / isolation & purification
  • Ultrasonic Waves

Substances

  • Triterpenes
  • Plant Extracts
  • Deep Eutectic Solvents