A Gram-stain-negative, rod-shaped, non-motile, aerobic, light-yellow-pigmented bacterium, designated as strain Y10T, was isolated from Lumnitzera racemosa leaf in Iriomote island mangrove forests in Japan. The 16S rRNA gene sequence analysis revealed that the isolate Y10T was affiliated with the family Flavobacteriaceae, and the sequence showed the highest sequence identity to that of Neptunitalea chrysea NBRC 110019T (97.2%) and others with below 96% sequence identity. The draft genome analysis revealed that its genomic size is 3.85 Mbp with G + C content 34.1%. The average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values between strain Y10T and Neptunitalea chrysea NBRC 110019T was 79.07%, 76.35% and 19.6%, respectively, which were below the thresholds for prokaryotic species delineation (95-96%, 85%, and 70%, respectively). MK-6 was the only menaquinone, and the major cellular fatty acids (> 10% of the total fatty acids) were iso-C15:0, iso-C17:0 3-OH, and summed feature 3 (comprised of C16:1ω7c-iso and C15:0 2-OH). Based on its distinct phylogenetic position and genotypic and phenotypic characteristics, the strain represents a novel species of the genus Neptunitalea, for which the name Neptunitalea lumnitzerae sp. nov. is proposed. The type strain is Y10T (= DSM 110280T = NBRC 115783T).
Keywords: Mangrove; Neptunitalea lumnitzerae sp.nov.; Phyllosphere; Lumnitzera reacemosa.
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