The covalent immobilization of proteins attracts considerable interest in the biomedical field due to its potential applications in biosensors, recombinant protein purification, and the development of personalized therapeutic carriers. In response to the demand for more cost-effective, time-efficient, and simpler protocols, photo-immobilization emerges as a technique that circumvents the limitations of conventional methods. This approach offers enhanced precision at the nanoscale level and facilitates device reusability, thereby aligning with current sustainability concerns. Photo-immobilization is versatile, as it can be applied to both 2D and 3D substrates. While some methods involve complex protocols using genetically engineered photosensitive linkers, more straightforward techniques rely on amino acid bonds, such as disulfide bonds, for covalent protein bonding. Photo-immobilization can be achieved with both ultraviolet (UV) and visible light. This systematic review examines literature from Scopus, PubMed, and Web of Science, offering insights into relevant studies and considerations for covalent protein immobilization, and presents photochemical approaches applicable to major protein types.
Keywords: UV light; biomedical applications; covalent bonds; photo‐immobilization; protein immobilization; visible light.
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