Maternal behavior is a complex form of social conduct exhibited, which directly influences the brain development and emotional behavior of offspring. Studies have shown that stress significantly impacts maternal behavior, with the 5-HT (serotonin) system playing a crucial role in this process OBJECTIVE: This study aims to explore the ameliorative effects of omega-3 fatty acid supplementation on maternal behavioral abnormalities induced by prenatal chronic restraint stress, and to analyze the molecular mechanisms involved METHODS: Sprague-Dawley rats were divided into control, stress, and stress+omega-3 (500 mg/kg) groups. Depressive behaviors were assessed using the sucrose preference test and forced swimming test. The regulatory effects of Omega-3 on maternal behavior following stress were analyzed by measuring 5-HT levels, 5-HT receptors, 5-HT1A receptor expression, downstream cAMP levels, inflammatory markers (IL-1β, TNF-α, and IL-6), and oxidative stress responses (MDA levels) RESULTS: The stress group exhibited significant reductions in maternal behaviors, such as delayed pup retrieval and decreased licking time. Omega-3 supplementation effectively improved these abnormalities, enhancing maternal care and reducing violent behaviors. Mechanistically, omega-3 supplementation increased 5-HT and receptor expression, reduced inflammation and oxidative stress, and promoted neuronal function recovery CONCLUSION: Omega-3 fatty acids can effectively mitigate the negative impact of chronic stress on maternal behavior. The underlying mechanisms involve the regulation of the 5-HT system and the reduction of neuroinflammation. This finding provides a theoretical basis for clinical interventions targeting stress-related maternal behavior disorders.
Keywords: 5-HT; Maternal behavior; Omega-3 fatty acid; Prenatal chronic restraint stress.
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