The current status and future prospects of the Synchrotron Radiation Protein Crystallography Core Facility at NSRRC: a focus on the TPS 05A, TPS 07A and TLS 15A1 beamlines

Chung Kuang Chou; Chien Chang Tseng; Cheng Hung Chiang; Yi Hui Chen; Yi Chun Liu; Chen Ying Huang; Chun Hsiung Chao; Chun Hsiang Huang

doi:10.1107/S1600577525000177

The current status and future prospects of the Synchrotron Radiation Protein Crystallography Core Facility at NSRRC: a focus on the TPS 05A, TPS 07A and TLS 15A1 beamlines

J Synchrotron Radiat. 2025 Mar 1;32(Pt 2):457-465. doi: 10.1107/S1600577525000177. Epub 2025 Feb 6.

Authors

Chung Kuang Chou¹, Chien Chang Tseng¹, Cheng Hung Chiang¹, Yi Hui Chen¹, Yi Chun Liu¹, Chen Ying Huang¹, Chun Hsiung Chao¹, Chun Hsiang Huang¹

Affiliation

¹ Protein Diffraction Group, Experimental Facility Division, National Synchrotron Radiation Research Center, 101 Hsin-Ann Road, Hsinchu Science Park, Hsinchu 300092, Taiwan.

Abstract

The Synchrotron Radiation Protein Crystallography Core Facility (SPXF) at the National Synchrotron Radiation Research Center has been pivotal in advancing structural biology research in Taiwan. Since the 1990s, Taiwan has invested in the development of synchrotron light sources, including the Taiwan Light Source and the Taiwan Photon Source, which have profoundly enhanced protein structure analysis capability. Through the high-performance beamlines, SPXF has enabled significant scientific achievements, contributing to disease research and drug development. The facility's move towards automation and its integration of advanced techniques, such as in situ serial synchrotron crystallography, underscore its commitment to meeting the evolving needs of the research community. The SPXF continues to foster innovation and collaboration, providing world-class resources for both domestic and international users.

Keywords: SPXF; Synchrotron Radiation Protein Crystallography Core Facility; Taiwan Light Source; Taiwan Photon Source; beamline automation; protein crystallography; serial synchrotron crystallography.

open access.

Publication types

Review

MeSH terms

Crystallography, X-Ray / instrumentation
Crystallography, X-Ray / methods
Protein Conformation
Proteins* / chemistry
Synchrotrons* / instrumentation
Taiwan

Substances

Proteins

Grants and funding

The funding for the SPXF is primarily provided by the National Science and Technology Council and the NSRRC, and the following sources of funding are acknowledged. From NSTC: the National Research Program for Genomic Medicine, the National Research Program for Biopharmaceuticals and the National Core Facility for Biopharmaceuticals. From NSRRC: Maintenance of High-Performance Biological Crystallography Experimental Station, Operation and Maintenance of Microcrystallography Facilities, and Operational and Maintenance of Micro-focused Protein Crystallography. Due to the numerous grant numbers, these will not be listed individually.