Tissue factor (coagulation factor III) is present in placental microvilli and cofractionates with microvilli membrane proteins

Placenta. 1985 Jan-Feb;6(1):5-12. doi: 10.1016/s0143-4004(85)80027-8.

Abstract

We have demonstrated that tissue factor is present in preparations of placental microvilli. The similarity of the procoagulant activities of microvilli and placental homogenates indicate that tissue factor is neither deficient nor enriched in microvilli relative to the bulk of placental tissue. On the basis of solubility in Triton X-100 and ammonium sulphate, and its affinity for phenyl-Sepharose, microvilli tissue factor is indistinguishable from that in whole placental homogenates.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Humans
  • Membrane Proteins / analysis
  • Microscopy, Electron
  • Microvilli / analysis
  • Microvilli / ultrastructure
  • Placenta / analysis*
  • Placenta / ultrastructure
  • Pregnancy
  • Pregnancy Proteins / analysis*
  • Thromboplastin / analysis*

Substances

  • Membrane Proteins
  • Pregnancy Proteins
  • Thromboplastin