A Clinical Evaluation of the Safety, Efficacy, and Tolerability of the Soulflower Rosemary Redensyl Hair Growth Serum, Tetragain™, in Healthy Female Subjects for the Treatment of Alopecia: Promoting Hair Growth and Reducing Gray Hair

Abstract

Introduction Alopecia and graying hair, common conditions influenced by aging, genetics, and environmental factors, often see limited success with traditional treatments. Rosemary extract, valued for its antioxidant and anti-inflammatory benefits, has gained attention for promoting hair growth and reducing graying. Redensyl™, a plant-based ingredient, supports hair follicle regeneration and enhances hair density. This study examines the effectiveness of Soulflower Rosemary Redensyl Hair Growth Serum (Tetragain™), which is formulated using Redensyl, Oryza sativa (rice) water extract , Salvia hispanica (chia) seed extract, rosemary oil, MelanoGray™, and AnaGain™, in promoting hair growth, enhancing follicular activity, and reducing graying. Methods This open-label, single-arm, prospective interventional study evaluated the safety, efficacy, and tolerability of a hair growth and anti-gray hair serum. Ethical approval was obtained, and participants provided informed consent. The study measured changes in hair growth rate, length, density, thickness, anagen:telogen (A:T) ratio, hair fall, graying severity, and scalp appearance using a phototrichogram (CASLite Nova, Catseye Systems & Solutions Pvt Ltd, Navi Mumbai, Maharashtra, India), the 60-second hair comb test, and dermatological evaluations. Consumer perception of the test treatment was evaluated using a questionnaire. Statistical analysis was conducted using IBM SPSS Statistics for Windows, Version 29.0.1.0 (Released 2023; IBM Corp., Armonk, New York, United States) and Microsoft Excel 2019 (Microsoft Corporation, Redmond, Washington, United States), with results reported at a 5% significance level. Results The study found that applying a test treatment led to a significant reduction in gray hairs and an improvement in hair growth over the study duration of 120 days after the use of the test treatment. Hair growth rate was enhanced by 46.71% on Day 90, hair length improved by 35.40% on Day 87, A:T Ratio improved by 48.26% on Day 90, hair density improved by 37.92%, hair thickness improved by 80.85% on Day 120, reduction in hair fall observed by 64.89% on Day 120, improvement in Graying Severity Score improved by 64.89% after the use of the test treatment, which was statistically significant (p-value of <0.001). The experience of the test was reported by all the subjects as 'effective' in improving hair growth and gray hairs and they were satisfied after use. No adverse effects were observed during the study. Conclusion The test treatment, Soulflower Rosemary Redensyl Hair Growth Serum (Tetragain), exhibited a favorable safety profile, with no reported adverse reactions, making it suitable for regular use in daily hair care routines. Its formulation effectively enhances hair density, reduces hair fall, and addresses early signs of graying, providing a clinically supported option for individuals seeking to improve hair health and maintain natural color. This formulation can normalize the hair growth cycle, thereby fostering an optimal environment for sustained hair growth.

Keywords: alopecia; gray hairs; hair density; hair fall; hair growth.