Background: NDM-1 has been frequently identified in Enterobacteriaceae strains from clinical samples, but its occurrence in Salmonella Typhimurium (S. Typhimurium) is uncommon. Moreover, reports on the transmission of a plasmid harboring NDM-1 between different species in clinical samples are rare.
Methods: Two S. Typhimurium strains and an Escherichia coli (E. coli) strain were isolated from a 14-month-old infant presenting with diarrhea. The resistance phenotypes were determined using the VITEK 2 Compact System. Genomic DNA was extracted from the isolated strains and whole genome sequencing was performed. The transmissibility of plasmids was validated by a conjugation experiment.
Results: The subsequently isolated S. Typhimurium and E. coli strains exhibited resistance to imipenem, and whole genomic analysis revealed that each strain harbored a plasmid harboring NDM-1 and Qnrs1 genes. The two plasmids were nearly identical based on their sequences and could transfer to recipient strains. A genomic region containing several genes encoding phosphoribosylanthranilate isomerase, bleomycin binding protein, subclass B1 metallo-beta-lactamase, and IS30-like element ISAba125 family transposase was surrounded by the two flanking IS26 elements.
Conclusion: The transfer of plasmid harboring NDM-1 between S. Typhimurium and E. coli strains within patients highlights the potential for widespread transmission between different species. Continuous monitoring these strains may be important for preventing extensive transmission of resistance genes.
Keywords: Escherichia coli; NDM-1; Salmonella Typhimurium; Subclass B1 metallo-beta-lactamase; Whole genome sequencing.
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