Overcoming kinetic barriers of remote electrochemiluminescence on boron-doped diamond via catalytic coreactant oxidation

Alessandro Fracassa; Chiara Mariani; Andrea Fiorani; Yasuaki Einaga; Conor F Hogan; Francesco Paolucci; Neso Sojic; Paul S Francis; Giovanni Valenti

doi:10.1039/d5cc00270b

Overcoming kinetic barriers of remote electrochemiluminescence on boron-doped diamond via catalytic coreactant oxidation

Chem Commun (Camb). 2025 Feb 27;61(19):3900-3903. doi: 10.1039/d5cc00270b.

Authors

Alessandro Fracassa¹, Chiara Mariani¹, Andrea Fiorani², Yasuaki Einaga², Conor F Hogan³, Francesco Paolucci^{1

4}, Neso Sojic⁵, Paul S Francis⁶, Giovanni Valenti¹

Affiliations

¹ Department of Chemistry "Giacomo Ciamician", Alma Mater Studiorum - University of Bologna, Via Gobetti 85, 40129 Bologna, Italy. g.valenti@unibo.it.
² Department of Chemistry, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan.
³ The Biomedical and Environmental Sensor Technology (BEST) Research Centre, Biosensors Program, La Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne, 3086, VIC, Australia.
⁴ ICMATE-CNR, Corso Stati Uniti 4, 35127 Padova, Italy.
⁵ University Bordeaux, CNRS, Bordeaux INP, ISM, UMR 5255, ENSMAC, 33607, Pessac, France.
⁶ Centre for Sustainable Bioproducts, Faculty of Science, Engineering and Built Environment, Deakin University, Geelong, Victoria 3220, Australia.

PMID: 39936237
DOI: 10.1039/d5cc00270b

Abstract

The effectiveness of boron-doped diamond (BDD) as a platform for electrochemiluminescence (ECL) bead-based bioassays is hindered by the sluggish rate of heterogeneous tri-n-propylamine (TPrA) oxidation. To address this, we investigate the ECL of Ru(II)-coated microbeads in the presence of a redox mediator, exploring the effect of applied potential and electrode surface terminations. Using a redox mediator, the ECL signal on BDD is enhanced by up to 46-fold.