Development of an efficient differentiation culture system of murine HSC into megakaryocytes

Abstract

Despite significant progress in the cultivation of hematopoietic stem cells (HSCs), the establishment of lineage-specific cell culture systems remains inadequately developed. This study compares the effects of recombinant human serum albumin (r-HSA) and polyvinyl alcohol (PVA) in serum-free culture systems on the differentiation of HSCs into multiple lineages. Both single-cell and multi-cellular culture systems are used, and differentiation is evaluated by flow cytometry and slides-based techniques under various cytokine conditions. Our results show that r-HSA strongly promotes differentiation into megakaryocytes (MKs) compared to PVA. The findings indicate that r-HSA outperforms PVA in supporting MK differentiation through both early expansion and later differentiation. This study provides insights into optimizing megakaryocyte generation and offers a more effective culture system for clinical applications.

Keywords: Hematopoietic stem cells; Megakaryocytes differentiation; PVA; Serum-free culture; r-HSA.