Impact of whey protein on lipid profiles: A systematic review and meta-analysis

Nutr Metab Cardiovasc Dis. 2025 Jun;35(6):103858. doi: 10.1016/j.numecd.2025.103858. Epub 2025 Jan 6.

Abstract

Aims: This research delved into a comprehensive examination and detailed analysis of the effects of whey protein (WP) supplementation on lipid profile in adults.

Data synthesis: Data used in this research was obtained from diverse clinical trials. Thorough searches were carried out on multiple electronic platforms including PubMed, Embase, Web of Science, Scopus, Google Scholar, and the Cochrane Library from their inception until May 2024. Random effects models were assessed and pooled data were determined as weighted mean differences (WMDs) with a 95 % confidence interval (CI).

Results: Overall, 20 randomized clinical trials (n = 1638 participants) met our inclusion criteria. The current meta-analysis demonstrates a significant reduction in TG (WMD: -12.21 mg/dL; %95CI: -20.16, -4.26; P = 0.003). Pooled analysis of 19 studies on HDL-c indicated a significant increase (WMD: 2.59 mg/dL; %95CI: 1.11, 4.07; P = 0.001).

Conclusions: We found that WP intake can improve TG and HDL-c significantly without significant effects on TC, and LDL-c levels. However, future well-designed with long duration RCTs is required on diverse populations to understand better the effects of these natural compounds and their constituents on lipid profile in adults.

Keywords: Lipid profile; Meta-analysis; Supplementation; Whey protein.

Publication types

  • Systematic Review
  • Meta-Analysis

MeSH terms

  • Adult
  • Aged
  • Biomarkers / blood
  • Dietary Supplements* / adverse effects
  • Dyslipidemias* / blood
  • Dyslipidemias* / diagnosis
  • Dyslipidemias* / epidemiology
  • Female
  • Humans
  • Lipids* / blood
  • Male
  • Middle Aged
  • Randomized Controlled Trials as Topic
  • Treatment Outcome
  • Triglycerides / blood
  • Whey Proteins* / administration & dosage
  • Whey Proteins* / adverse effects

Substances

  • Whey Proteins
  • Biomarkers
  • Lipids
  • Triglycerides