A method of isolating nick-translated DNA by subsequent separation on low-melting-temperature agarose

Anal Biochem. 1985 Apr;146(1):23-7. doi: 10.1016/0003-2697(85)90389-6.


The use of nick translation of cloned DNA segments followed by separation on low-melting-temperature agarose has been used to obtain multiple radiolabeled DNA probes from a single nick-translation procedure. This technique avoids gel matrix inhibition of enzymatic reactions. Examples of the utility of this procedure are presented and the advantages and drawbacks discussed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Autoradiography
  • Chemical Phenomena
  • Chemistry
  • Cloning, Molecular
  • Electrophoresis, Agar Gel
  • Nucleic Acid Hybridization*
  • Oncogenes
  • Protein Biosynthesis*
  • Thermodynamics