The steroidic fraction from the ethereal extract of the aereal parts of Erythraea centaurium Rafn. has showed the presence of the following sterols: beta-sitosterol, stigmasterol, campesterol, brassicasterol and delta 7 stigmastenol, isolated by H.P.L.C. and identified by G.L.C., 1H-N.M.R. and MS. Then the free amino-acids from the aqueous extract of E. centaurium were determined.