Complete genome sequencing and probiotic trait analysis of Lacticaseibacillus rhamnosus LR110, a human stool isolate from the NORDBIOTIC collection

A Szczepankowska; B Cukrowska; T Aleksandrzak-Piekarczyk

doi:10.1128/mra.01116-24

Complete genome sequencing and probiotic trait analysis of Lacticaseibacillus rhamnosus LR110, a human stool isolate from the NORDBIOTIC collection

Microbiol Resour Announc. 2025 Mar 11;14(3):e0111624. doi: 10.1128/mra.01116-24. Epub 2025 Feb 24.

Authors

A Szczepankowska¹, B Cukrowska², T Aleksandrzak-Piekarczyk¹

Affiliations

¹ Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
² Department of Pathomorphology, The Children Memorial Health Institute, Warsaw, Poland.

Abstract

We present the complete genome of Lacticaseibacillus rhamnosus strain LR110, a human stool isolate from the NORDBIOTIC strain collection. The genome consists of a 2,867,184-bp chromosome with 46.8% GC content. Genomic analysis revealed genes related to thiamine salvage, lactose metabolism, and putrescine biosynthesis, providing insight into the strain's potential probiotic properties.

Keywords: Lactocaseibacillus rhamnosus; anti-obesity; bacteriocins; gut homeostasis; lactobacilli; lactose metabolism; ornithine decarboxylase; probiotics; putrescine biosynthesis; vitamin B1.