The accumulation of biofilms can potentially be very costly in terms of damage to mechanical systems and health impact on the human body. Space travel, especially long-term space travel, compounds the complications that arise from the accumulation of biofilms because of the lack of access to resources. This study investigates the ability of polyampholyte copolymer thin films to reduce bacteria adhesion in microgravity. Copolymer systems of [2-(acryloyloxy)ethyl] trimethylammonium chloride (TMA) and 2-carboxyethyl acrylate (CAA) and TMA and 3-sulfopropyl methacrylate potassium salt (SA) have previously shown resistance to bacteria adhesion under gravity-impacted conditions. However, their performance under microgravity conditions has never been evaluated. A self-contained payload was designed around multiple constraints to evaluate the ability of the TMA/CAA and TMA/SA thin film coatings to reduce the adhesion and biofilm formation of Staphylococcus epidermidis on aluminum test coupons in microgravity in an experiment conducted onboard the International Space Station (ISS). An Earth-based, gravity-impacted study was completed in parallel with the ISS experiment. The samples were then analyzed on the macroscale using photography and the microscale using confocal microscopy imaging to determine biofilm formation and bacteria attachment, respectively. The percentage of each sample covered by bacteria and/or biofilm was characterized and compared amongst the coating types and gravity exposure conditions. The TMA/SA coatings showed the lowest levels of bacteria adhesion and biofilm formation overall. The TMA/CAA coatings showed the largest reduction in bacteria adhesion and biofilm formation when comparing adhesion between the microgravity- and gravity-impacted samples. Therefore, both the copolymers demonstrate promise for bacteria-resistant coatings in microgravity.
Keywords: International Space Station; S. epidermidis; microgravity; nonfouling; polyampholytes.