Conformational requirements of tobacco mosaic virus RNA for aminoacylation and adenylation

Nucleic Acids Res. 1985 Jan 25;13(2):347-54. doi: 10.1093/nar/13.2.347.

Abstract

The RNA conformational requirements for both aminoacylation and adenylation emerging from our studies performed using the valine- and the tyrosine-accepting plant viral RNAs are now strongly supported by the histidine-accepting tobacco mosaic virus RNA: an 'L'-shaped conformation is recognized by the aminoacyl-tRNA synthetase whereas only the aminoacyl RNA domain (equivalent in tRNAs to the continuous helix formed by the acceptor stem and the T stem and loop) interacts with the tRNA nucleotidyltransferase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acyl-tRNA Synthetases / metabolism*
  • Molecular Weight
  • Nucleic Acid Conformation
  • RNA Nucleotidyltransferases / metabolism*
  • RNA, Viral* / metabolism
  • Structure-Activity Relationship
  • Substrate Specificity
  • Tobacco Mosaic Virus / metabolism*

Substances

  • RNA, Viral
  • RNA Nucleotidyltransferases
  • tRNA nucleotidyltransferase
  • Amino Acyl-tRNA Synthetases