Selective immunoprecipitate identification using monoclonal anti-rabbit IgG

Anal Biochem. 1985 Feb 15;145(1):148-50. doi: 10.1016/0003-2697(85)90340-9.

Abstract

A monoclonal mouse antibody directed against rabbit IgG has been conjugated with horseradish peroxidase and used to identify immunoprecipitates which contain rabbit antibodies. By combining a specific rabbit antisera with a general antiserum from another species (e.g., goat antiserum against human serum), immunoprecipitates containing the antigen(s) recognized by the rabbit antibodies have been selectively identified by colorimetric development of peroxidase activity. Since the monoclonal antibody is specific for rabbit IgG and nonprecipitating, the peroxidase conjugate can be included in the agarose with the primary antisera.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Chemical Precipitation / methods*
  • Goats
  • Horseradish Peroxidase
  • Humans
  • Immunochemistry / methods*
  • Immunoelectrophoresis, Two-Dimensional
  • Immunoglobulin G*
  • Rabbits

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin G
  • Horseradish Peroxidase