Influence of FOXP3 rs2280883 and rs3761548 Variants on IL-10 and TGF-β1 Serum Levels and Plaque Psoriasis Risk in the Mexican Population

Int J Mol Sci. 2025 Feb 20;26(5):1789. doi: 10.3390/ijms26051789.

Abstract

Plaque psoriasis (PP) is a chronic immune-mediated skin disorder with a genetic basis, characterized by abnormal T-cell responses. This study investigated the role of FOXP3 gene variants rs2280883 and rs3761548 in T-cell regulation through their effects on IL-10 and TGF-β1 cytokine levels and their association with PP risk. A case-control study was conducted, including 101 individuals with PP and 106 healthy controls from the Mexican population. Genotyping of FOXP3 variants was performed using PCR-RFLP, and cytokine levels were measured with ELISA kits. Significant differences in allele and genotype frequencies of the rs2280883 variant were observed between PP patients and controls, suggesting an association with an increased risk of PP. IL-10 levels were found to be elevated in PP patients, regardless of FOXP3 gene variants, indicating that cytokine dysregulation in PP may involve alternative pathways independent of FOXP3-mediated regulatory T-cell (Treg) function. No significant differences were detected in TGF-β1 levels or rs3761548 genotype frequencies across the study groups. In conclusion, the rs2280883 variant in the FOXP3 gene is significantly associated with a higher risk of developing PP in the Mexican population, while dysregulated IL-10 levels suggest a complex cytokine interaction beyond Treg activity.

Keywords: FOXP3 variants; IL-10; TGF-?1; Tregs; plaque psoriasis.

MeSH terms

  • Adult
  • Case-Control Studies
  • Female
  • Forkhead Transcription Factors* / genetics
  • Gene Frequency
  • Genetic Predisposition to Disease
  • Genotype
  • Humans
  • Interleukin-10* / blood
  • Male
  • Mexico / epidemiology
  • Middle Aged
  • Polymorphism, Single Nucleotide*
  • Psoriasis* / blood
  • Psoriasis* / epidemiology
  • Psoriasis* / genetics
  • T-Lymphocytes, Regulatory / immunology
  • Transforming Growth Factor beta1* / blood

Substances

  • Forkhead Transcription Factors
  • FOXP3 protein, human
  • Transforming Growth Factor beta1
  • Interleukin-10
  • IL10 protein, human
  • TGFB1 protein, human