Association of carboxyhemoglobin and methemoglobin levels with hemolysis during extracorporeal membrane oxygenation

Int J Artif Organs. 2025 Apr;48(4):250-257. doi: 10.1177/03913988251326398. Epub 2025 Mar 13.

Abstract

Introduction: Hemolysis, characterized by increased carboxyhemoglobin (COHb) and methemoglobin (MetHb) levels, is a complication of extracorporeal membrane oxygenation (ECMO).

Methods: This prospective single-center study aimed to investigate the correlation between COHb and MetHb levels and hemolysis during ECMO. This study included 32 patients requiring ECMO for circulatory or respiratory failure. Plasma-free hemoglobin (pfHb), COHb, and MetHb levels were measured simultaneously within 6 h of ECMO induction, daily during ECMO, within 6 h after decannulation, and 2 days after decannulation unless death occurred before. Patients were classified into hemolysis and non-hemolysis groups based on whether the maximum pfHb level during ECMO was ⩾50 mg/dL.

Results: No significant difference in maximum COHb levels during ECMO (COHbECMO) was observed between the hemolysis and non-hemolysis groups (2.15% [interquartile range (IQR) = 1.83, 2.60] vs 1.65% [IQR = 1.40, 2.10], p = 0.159). However, maximum MetHb levels during ECMO (MetHbECMO) were significantly higher in the hemolysis group (1.35% [IQR = 1.12, 1.78] vs 1.10% [IQR = 0.90, 1.37], p = 0.045). The Spearman's correlation coefficients for COHbECMO and MetHbECMO were 0.39 (95% confidence interval [CI] = 0.456-0.649) and 0.66 (95% CI = 0.404-0.820), respectively.

Conclusion: Elevated MetHb levels in patients undergoing ECMO may be associated with hemolysis.

Keywords: ECMO; carboxyhemoglobin; hemolysis; methemoglobin; plasma free hemoglobin.

MeSH terms

  • Adult
  • Carboxyhemoglobin* / analysis
  • Carboxyhemoglobin* / metabolism
  • Extracorporeal Membrane Oxygenation* / adverse effects
  • Female
  • Hemolysis*
  • Humans
  • Male
  • Methemoglobin* / analysis
  • Methemoglobin* / metabolism
  • Middle Aged
  • Prospective Studies
  • Respiratory Insufficiency* / blood
  • Respiratory Insufficiency* / therapy

Substances

  • Methemoglobin
  • Carboxyhemoglobin