Immunity gene silencing increases transient protein expression in Nicotiana benthamiana

Isobel L Dodds; Emma C Watts; Mariana Schuster; Pierre Buscaill; Yasin Tumas; Nicholas J Holton; Shijian Song; Johannes Stuttmann; Matthieu H A J Joosten; Tolga Bozkurt; Renier A L van der Hoorn

doi:10.1111/pbi.70005

Immunity gene silencing increases transient protein expression in Nicotiana benthamiana

Plant Biotechnol J. 2025 May;23(5):1768-1770. doi: 10.1111/pbi.70005. Epub 2025 Mar 13.

Affiliations

¹ The Plant Chemetics Laboratory, Department of Biology, University of Oxford, Oxford, UK.
² Department of Life Sciences, Imperial College London, London, UK.
³ Leaf Expression Systems, Norwich Research Park, Norwich, UK.
⁴ Aix Marseille University, CEA, CNRS, BIAM, UMR7265, LEMiRE (Microbial Ecology of the Rhizosphere), Saint-Paul lez Durance, France.
⁵ Laboratory of Phytopathology, Wageningen University and Research, Wageningen, The Netherlands.

PMID: 40083115
PMCID: PMC12018844
DOI: 10.1111/pbi.70005

Abstract

Keywords: Agrobacterium tumefaciens; GFP fluorescence; Nicotiana benthamiana; agroinfiltration; transient expression; virus‐induced gene silencing.

Grants and funding

BB/R017913/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom
BB/S003193/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom
DDT00060/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom
DDT00230/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom
101019324/ERC_/European Research Council/International