Impact of Vitamin E-Coated Membrane Hemodiafilter on Serum Albumin Redox State in the Acute Kidney Injury Pig Hemodialysis Model

Abstract

Background: Several studies have evaluated the biocompatibility of dialysis membranes. The use of vitamin E-coated membranes has been reported multilaterally in in vitro and clinical studies. Nevertheless, the effect of vitamin E-coated membranes on the redox state of serum albumin, which forms the largest fraction of reactive sulfhydryl groups, has not been reported.

Methods: Hemodiafiltration (HDF) with and without a vitamin E-coated hemodiafilter (V-RA^TM group and ABH^TM groups, respectively) was performed in an acute kidney injury pig model to determine whether changes in the serum albumin, the oxidized albumin (OxiALB), and the reduced albumin (RedALB) levels differ between the two groups.

Results: Analyses were conducted 22-24 times in the V-RA^TM group and 16-18 times in the ABH^TM group, excluding missing data. The serum albumin levels decreased in both groups after nephrectomy; however, the decrease observed in the V-RA^TM group was significantly lesser than that in the ABH^TM group. RedALB levels were significantly higher in the V-RA^TM group; in contrast, OxiALB levels did not differ between the two groups. A significant positive correlation was observed between the serum albumin and RedALB levels.

Conclusions: The present study demonstrated that HDF performed using a vitamin E-coated hemodiafilter effectively minimized the reduction in serum albumin and RedALB levels compared to the vitamin E-non-coated hemodiafilter in an acute kidney injury pig model.

Keywords: acute kidney injury; hemodiafilter; oxidative stress; reduced albumin; vitamin E‐coated.