A complete genome of an obligately lytic Pseudomonas aeruginosa bacteriophage, Minga-mokiny 4

Phoebe G Carr; Kak-Ming Ling; Joshua J Iszatt; Wee Peng Poh; Erika N Sutanto; Renee N Ng; Barbara J Chang; Stephen M Stick; Anthony Kicic; WAERP; AREST CF

doi:10.1128/mra.01289-24

A complete genome of an obligately lytic Pseudomonas aeruginosa bacteriophage, Minga-mokiny 4

Microbiol Resour Announc. 2025 Apr 10;14(4):e0128924. doi: 10.1128/mra.01289-24. Epub 2025 Mar 20.

Authors

Phoebe G Carr¹, Kak-Ming Ling^{1

2}, Joshua J Iszatt¹, Wee Peng Poh³, Erika N Sutanto¹, Renee N Ng¹, Barbara J Chang⁴, Stephen M Stick⁵, Anthony Kicic^{1

2

5

6}; WAERP^{1

7}; AREST CF^{1

5

8

9}

Affiliations

¹ Wal-Yan Respiratory Research Centre, The Kids Research Institute Australia, The University of Western Australia, Perth, Western Australia, Australia.
² Occupation, Environment and Safety, School of Population Health, Curtin University, Perth, Western Australia, Australia.
³ School of Biomedical Sciences, University of Western Australia, Perth, Western Australia, Australia.
⁴ The Marshall Centre for Infectious Diseases Research and Training, School of Biomedical Sciences, The University of Western Australia, Perth, Western Australia, Australia.
⁵ Department of Respiratory and Sleep Medicine, Perth Children's Hospital, Nedlands, Western Australia, Australia.
⁶ Centre for Cell Therapy and Regenerative Medicine, School of Medicine and Pharmacology, The University of Western Australia and Harry Perkins Institute of Medical Research, Perth, Western Australia, Australia.
⁷ St. John of God Hospital, Subiaco, Perth, Western Australia, Australia.
⁸ Murdoch Children's Research Institute, Melbourne, Victoria, Australia.
⁹ Department of Pediatrics, University of Melbourne, Melbourne, Victoria, Australia.

Abstract

We report the isolation of a bacteriophage with obligately lytic activity against Pseudomonas aeruginosa from wastewater. The reported phage, Minga-mokiny 4, appears to belong to the Schitoviridae family, is of the Litunavirus genus, and has a 72,362-bp genome. No known genes associated with lysogeny, bacterial resistance, or virulence were predicted.

Keywords: Pseudomonas aeruginosa; bacteriophages; genomes; phage.