Successful Cultivation of Spirochetes From Skin Lesions of Patients With Erythema Chronicum Migrans Afzelius and Acrodermatitis Chronica Atrophicans

Acta Pathol Microbiol Immunol Scand B. 1985 Apr;93(2):161-3. doi: 10.1111/j.1699-0463.1985.tb02870.x.

Abstract

Spirochetes could be cultivated from 9 out of 13 skin biopsies from patients with erythema chronicum migrans Afzelius (ECMA) and from 2 out of 5 biopsies from patients with acrodermatitis chronica atrophicans (ACA) by using a newly modified serumless Kelly's medium. The different spirochete strains grew best at a low oxygen tension. Attempts to grow spirochetes from blood and cerebrospinal fluid failed. The cultivation of spirochetes from secondary ECMA lesions favours the presumption that a spirochetemia may occur in ECMA. The isolation of spirochetes from an ACA patient who had a disease duration of greater than 10 years proves that the spirochetes may survive in the human body for a considerable time.

MeSH terms

  • Acrodermatitis / etiology*
  • Acrodermatitis / microbiology
  • Arachnid Vectors
  • Erythema / etiology*
  • Erythema / microbiology
  • Humans
  • Spirochaetales / isolation & purification*
  • Spirochaetales Infections / etiology*
  • Spirochaetales Infections / transmission
  • Ticks