Microquantitative analysis of neutral and amino sugars as fluorescent pyridylamino derivatives by high-performance liquid chromatography

Anal Biochem. 1985 Mar;145(2):245-50. doi: 10.1016/0003-2697(85)90357-4.

Abstract

A method for the quantitation of picomole amounts of neutral and amino sugars in glycoconjugates was developed. Glycoconjugates were hydrolyzed with a mixture of equal amounts of 4 M trifluoroacetic acid and 4 M hydrochloric acid, and the free amino groups were acetylated. Sugars were coupled with 2-aminopyridine. After the excess reagents were removed by gel-permeation high-performance liquid chromatography, the fluorescent pyridylamino derivatives of sugars were separated and quantified by high-performance liquid chromatography on a reversed-phase column. This method allowed the determination of 0.01-10 nmol of sugars. About 100 pmol of several glycoconjugates were analyzed by the present method, with satisfactory results.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Sugars / analysis*
  • Aminopyridines*
  • Carbohydrates / analysis*
  • Chromatography, High Pressure Liquid
  • Hydrolysis
  • Microchemistry
  • Spectrometry, Fluorescence

Substances

  • Amino Sugars
  • Aminopyridines
  • Carbohydrates
  • alpha-aminopyridine