Genome Sequences of Cluster E Mycobacteriophages Xandras and BigBubba

Ashley Gledhill; Gabriel S Gooden; Janelle Aguazul; Aanyaa Arora; Ava R Blackledge; Michael H Bucks; Tessa Carby; Andrew Cu; Jacob Fulkerson; Chealsea W Gachagua; Elly Grogan; Haley H Hanson; Gillian N Johnston; Lola S Norman; Lindsey M Oak; Grant J Oller; Het Parekh; Sakshi Patel; Sydney Putnam; Grant Spalding; Jacob Thomas; Patrick Wallace; Claire A Rinehart; Rodney A King

doi:10.17912/micropub.biology.001460

Genome Sequences of Cluster E Mycobacteriophages Xandras and BigBubba

MicroPubl Biol. 2025 Mar 13:2025:10.17912/micropub.biology.001460. doi: 10.17912/micropub.biology.001460. eCollection 2025.

Authors

Ashley Gledhill^#¹, Gabriel S Gooden^#¹, Janelle Aguazul^#¹, Aanyaa Arora^#¹, Ava R Blackledge^#¹, Michael H Bucks^#¹, Tessa Carby^#¹, Andrew Cu^#¹, Jacob Fulkerson^#¹, Chealsea W Gachagua^#¹, Elly Grogan^#¹, Haley H Hanson^#¹, Gillian N Johnston^#¹, Lola S Norman^#¹, Lindsey M Oak^#¹, Grant J Oller^#¹, Het Parekh^#¹, Sakshi Patel^#¹, Sydney Putnam^#¹, Grant Spalding^#¹, Jacob Thomas^#¹, Patrick Wallace^#¹, Claire A Rinehart¹, Rodney A King¹

Affiliation

¹ Biology Department, Western Kentucky University, Bowling Green, Kentucky, United States.

^# Contributed equally.

Abstract

Bacteriophages Xandras and BigBubba were isolated on Mycobacterium smegmatis mc ² 155 from enriched soil samples. Xandras' genome length is 75,179 bp with 144 predicted protein-coding genes and two tRNAs. BigBubba's genome length is 75,006 bp with 147 predicted protein-coding genes and two tRNAs. Each genome contains a cyclic oligonucleotide sequestering protein (CBASS antagonist).