Production and characterization of [14C]protein A, a long-lived immunological reagent

J Immunol Methods. 1985 Aug 2;81(2):239-43. doi: 10.1016/0022-1759(85)90209-1.

Abstract

A procedure for the production of [14C]protein A is described which involves reductive methylation of lysine residues with [14C]formaldehyde and NaCNBH3. The binding of [14C]protein A to IgG is apparently unaltered, as determined by competitive binding studies. The use of [14C]protein A may be preferred to that of 125I-protein A when a radioactive label with a long half-life is desirable.

MeSH terms

  • Carbon Radioisotopes
  • Immunoglobulin G / analysis
  • Staphylococcal Protein A* / chemical synthesis
  • Time Factors

Substances

  • Carbon Radioisotopes
  • Immunoglobulin G
  • Staphylococcal Protein A