Neuroprotective effects of Garcinia kola and curcumin on diabetic transected sciatic nerve

Abstract

The growing interest in peripheral nerve regeneration and developing post-traumatic repair methods under diabetes was the impetus for this study, which aims to investigate the effect of curcumin andGarcinia kola(GK) on the transected and diabetic sciatic nerves. Thirty-five male Wistar albino rats were used. The animals were divided into five groups; each consisted of seven rats. The sciatic nerve was transected in all groups of rats except the control (Cont) group, which underwent no treatment. In the transected animals, a 10 mm nerve stump was removed from the 2 cm distal to the sciatic notch. The external jugular vein was used as a conduit to repair the gap between the two ends of the sciatic nerve. Diabetes was induced in the transected + diabetes mellitus (T + DM), the transected + diabetes mellitus + GK (T + DM + GK), and the transected + diabetes mellitus + Curcumin (T + DM + Cur) groups except for the sham group. A dose of 300 mg kg^-1d^-1of curcumin dissolved in olive oil was administered to the T + DM + Cur group (via oral gavage every day for 28 d) and 200 mg kg^-1d^-1of GK to the T + DM + GK group (via oral gavage every day for 7 d). All animals were sacrificed after three months. Stereological analysis and functional and microscopic evaluations were done to evaluate the sciatic nerve regeneration and function. In the T + DM + GK and the sham groups, the number of axons increased. A slight improvement in the axonal area in the T + DM + Cur and the sham groups was also observed, and an increase in the myelin sheath thickness was found in the T + DM + GK and the sham group. When the SFI test results were evaluated, it was seen that GK had a stronger effect than curcumin in terms of functional regeneration. Additionally, no significant difference was observed between T + DM and Cont groups when the electrophysiological results were examined. The study showed GK's efficiency in treating diabetic peripheral nerve regeneration.

Keywords: Garcinia kola; curcumin; diabetes; sciatic nerve; stereology; transection.