Detection of the c-myc oncogene product in testicular cancer

Br J Cancer. 1985 Aug;52(2):171-6. doi: 10.1038/bjc.1985.174.


A set of monoclonal antibodies was constructed by immunising mice with peptide fragments of the c-myc oncogene product. One such antibody, Myc 1-6E10 was shown to bind to a 62,000 dalton protein identifiable with the c-myc product (p62c-myc). The antigen recognised was not destroyed by paraffin wax embedding. Myc 1-6E10 was used to characterise the distribution of p62c-myc in archival testicular tumour material. Normal testes expressed only small amounts of p62c-myc. Seminomas showed increased nuclear and cytoplasmic staining. Undifferentiated teratoma showed little activity, whereas p62c-myc was abundant in the nuclei of differentiating epithelial structures, yolk sacs and embryoid bodies. Only small amounts of p62c-myc were seen in the tumours of 5 patients who subsequently died from their disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Cell Transformation, Neoplastic / metabolism
  • Dysgerminoma / metabolism
  • Humans
  • Male
  • Neoplasm Proteins / metabolism*
  • Oncogenes*
  • Protein Biosynthesis*
  • Teratoma / metabolism
  • Testicular Neoplasms / genetics
  • Testicular Neoplasms / metabolism*
  • Testis / metabolism


  • Antibodies, Monoclonal
  • Neoplasm Proteins