Exosomes from human umbilical cord mesenchymal stem cells promote the growth of human hair dermal papilla cells

PLoS One. 2025 Apr 30;20(4):e0320154. doi: 10.1371/journal.pone.0320154. eCollection 2025.

Abstract

Human hair dermal papilla cells (HHDPCs) play a significant role in hair growth. This study found that human umbilical cord mesenchymal stem cell-derived exosomes (UC-MSC-Es) effectively enhanced cell growth of HHDPCs. UC-MSC-Es has a size range of 30-180 nm and expression of CD9, CD63, CD81, CD73, and TSG101. UC-MSC-Es significantly increased cell populations of HHDPCs in the S and G2/M phases. UC-MSC-Es also increased the expression of cell cycle-related proteins, β-catenin, and cyclin D1. Further mechanistic studies demonstrated that UC-MSC-Es promoted the phosphorylation of Akt and GSK-3β, and the inhibition of PI3K and Akt reduced the proliferative effects of UC-MSC-Es. Collectively, these findings suggest that UC-MSC-Es have a potential effect in treating hair loss through modulating PI3K and Akt-dependent pathways in HHDPCs.

MeSH terms

  • Cell Cycle
  • Cell Proliferation
  • Cells, Cultured
  • Cyclin D1 / metabolism
  • Dermis* / cytology
  • Exosomes* / metabolism
  • Glycogen Synthase Kinase 3 / metabolism
  • Glycogen Synthase Kinase 3 beta
  • Hair Follicle* / cytology
  • Hair Follicle* / metabolism
  • Hair* / cytology
  • Hair* / growth & development
  • Humans
  • Mesenchymal Stem Cells* / cytology
  • Mesenchymal Stem Cells* / metabolism
  • Phosphatidylinositol 3-Kinases / metabolism
  • Phosphorylation
  • Proto-Oncogene Proteins c-akt / metabolism
  • Signal Transduction
  • Umbilical Cord* / cytology
  • beta Catenin / metabolism

Substances

  • Proto-Oncogene Proteins c-akt
  • Phosphatidylinositol 3-Kinases
  • beta Catenin
  • Glycogen Synthase Kinase 3 beta
  • Glycogen Synthase Kinase 3
  • Cyclin D1
  • GSK3B protein, human