A Robust Protocol for Pluripotent Stem Cell Modeling of 3D Chamber-Like Cardiac Organoids

Michel Pucéat

doi:10.1007/978-1-0716-4530-7_7

A Robust Protocol for Pluripotent Stem Cell Modeling of 3D Chamber-Like Cardiac Organoids

Methods Mol Biol. 2025:2924:93-100. doi: 10.1007/978-1-0716-4530-7_7.

Author

Michel Pucéat¹

Affiliation

¹ INSERM U1263, INRAE U1260 C2VN Cardiovascular and Nutrition Center, Aix-Marseille University, Marseille, France. michel.puceat@inserm.fr.

PMID: 40307637
DOI: 10.1007/978-1-0716-4530-7_7

Abstract

The formation of cardiac organoids brings a new 3D model of cardiac development. The organoid is also very helpful in screening drug efficiency or toxicity. Inspired by cardiac biology of development, a step-by-step protocol is described to generate cardiac chamber-like organoids from pluripotent stem cells. A few examples of functional tests and the use of these organoids for drug toxicity are reported.

Keywords: Cardiac development; Cardiogenesis; Drug screening; Heart; Organogenesis.

MeSH terms

Animals
Cell Culture Techniques / methods
Cell Culture Techniques, Three Dimensional* / methods
Cell Differentiation
Heart*
Humans
Induced Pluripotent Stem Cells / cytology
Myocytes, Cardiac / cytology
Organoids* / cytology
Organoids* / drug effects
Pluripotent Stem Cells* / cytology