Rapid purification of HBsAg from human plasma

J Virol Methods. 1985 Jul;11(3):225-30. doi: 10.1016/0166-0934(85)90111-9.

Abstract

A method is described for the rapid isolation of purified HBsAg from infected donor plasma. This method uses polyethylene glycol precipitation and a single isopycnic ultracentrifugation step in which the gradient is formed in situ. It works equally well using a small swingout rotor or a zonal rotor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Centrifugation, Isopycnic
  • Centrifugation, Zonal
  • Enzyme-Linked Immunosorbent Assay
  • Hepatitis B Surface Antigens / isolation & purification*
  • Humans
  • Plasma / immunology*
  • Plasma / microbiology
  • Polyethylene Glycols
  • Specimen Handling

Substances

  • Hepatitis B Surface Antigens
  • Polyethylene Glycols