Background and aims: Covert hepatic encephalopathy (CHE) is clinically underrecognized. Using MRI, we explored the relationship between functional connectivity as a marker of executive dysfunction and the blood-brain barrier permeability marker K TRANS in a cross-sectional cohort of CHE, no hepatic encephalopathy (NHE), and healthy controls (HCs).
Approach and results: This study was a single-center prospective cohort study conducted between 2018 and 2021. CHE was diagnosed using the Psychometric Hepatic Encephalopathy Score (PHES) with an abnormality threshold of <-4. Blood-brain barrier permeability was determined using MRI K TRANS , based on cerebral efflux of gadolinium from blood plasma to the extravascular space. Resting-state functional MRI determined the intrinsic dynamics of regions of the brain.Three cohorts were recruited: cirrhosis and CHE (n=17); cirrhosis with NHE (n=13); and HCs (n=10). There was a significant negative correlation ( r =-0.59, p =0.003) between K TRANS and components of the Default Mode Network (DMN). Group-level ANOVA ( F test) revealed a significant difference between groups in functional connectivity within the Salience Network (SN) (cluster-level significance, F (4, 62)=8.45, pcorrected =0.000005) and the DMN (cluster-level significance, F (4, 62)=6.20, pcorrected =0.0004). The analysis revealed significant differences in regional homogeneity between the CHE and HCs.
Conclusions: There was a strong association between disrupted blood-brain barrier integrity and attenuated functional connectivity, with reductions in functional connectivity within the DMN and Frontoparietal Network (FPN), circuits closely linked to executive control. This attenuation was evident both in local regional and in distant connectivity within these networks.
Keywords: MRI; blood–brain barrier; cirrhosis; covert; hepatic encephalopathy.
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