Plasma-based proteomics analysis of molecular pathways in canine diabetes mellitus after astaxanthin supplementation

PLoS One. 2025 May 7;20(5):e0321509. doi: 10.1371/journal.pone.0321509. eCollection 2025.

Abstract

The hyperglycemic state in diabetes mellitus induces oxidative stress and inflammation, contributing to diabetic tissue damage and associated complications. Astaxanthin, a potent antioxidant carotenoid, has been investigated for its potential to prevent and manage diabetes across various species; however, its effect on client-owned dogs remains poorly studied. This study explored the impact of astaxanthin supplementation on canine diabetes mellitus using a proteomics approach. A total of 18 client-owned dogs were enrolled: 6 dogs with diabetes mellitus and 12 clinically healthy dogs. The diabetic dogs received their standard treatment regimen along with daily oral supplementation of 12 mg of astaxanthin (1.5-2.4 mg/kg) for 90 days. Plasma samples were collected at the beginning and end of the study period for proteomics analysis. After astaxanthin supplementation, significant alterations in the expression of proteins associated with the complement system, coagulation cascade, JAK-STAT signaling, and protein kinase C signaling (all of which contribute to inflammation and oxidative stress) were observed. Astaxanthin exhibited potential for reducing diabetes-associated complications, such as insulin resistance, vascular dysfunction, nephropathy, and cardiac issues, even though it did not affect clinical parameters (hematology, plasma biochemistry, blood glucose, and serum fructosamine). These findings suggest that astaxanthin may be a valuable complementary therapy for managing diabetes-related complications in canines.

MeSH terms

  • Animals
  • Antioxidants
  • Blood Glucose
  • Diabetes Mellitus* / blood
  • Diabetes Mellitus* / drug therapy
  • Diabetes Mellitus* / metabolism
  • Diabetes Mellitus* / veterinary
  • Dietary Supplements*
  • Dog Diseases* / blood
  • Dog Diseases* / drug therapy
  • Dog Diseases* / metabolism
  • Dogs
  • Female
  • Male
  • Oxidative Stress / drug effects
  • Proteomics* / methods
  • Signal Transduction / drug effects
  • Xanthophylls / administration & dosage
  • Xanthophylls / pharmacology
  • Xanthophylls / therapeutic use

Substances

  • astaxanthine
  • Xanthophylls
  • Blood Glucose
  • Antioxidants