Seasonality of medically attended norovirus gastroenteritis and its association with climatic factors within an US integrated healthcare system, 2016-2019

PLoS One. 2025 May 9;20(5):e0318077. doi: 10.1371/journal.pone.0318077. eCollection 2025.

Abstract

Background: While acute gastroenteritis (AGE) occurs year-round, norovirus has a winter seasonality in the United States.

Objective: We analyzed norovirus seasonality within a US integrated healthcare delivery system from 2016-2019.

Methods: Electronic medical records were collected for acute gastroenteritis (AGE) encounters with specific ICD-9/10 codes or clinical stool testing. Norovirus percent positivity was calculated as the 8-week centered rolling average. Temperature and absolute humidity data were measured via weather station. The relationship between these factors and weekly norovirus episodes were modeled via negative binomial models.

Results: From 2016-2019, there were 198,181 AGE episodes reported; among the 18,998 episodes tested, 892 (5%) were norovirus positive. Norovirus percent positivity peaked in epidemiologic week 7 at 9%. Two negative binomial models showed significant inverse relationships between weekly number of norovirus episodes and both temperature and absolute humidity.

Conclusion: Norovirus AGE exhibited winter seasonality from 2016-2019, associated with lower temperatures and humidity. Understanding this seasonality may help predict peak transmission periods and their impact on healthcare resources.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Caliciviridae Infections* / epidemiology
  • Caliciviridae Infections* / virology
  • Child
  • Child, Preschool
  • Climate
  • Delivery of Health Care, Integrated
  • Female
  • Gastroenteritis* / epidemiology
  • Gastroenteritis* / virology
  • Humans
  • Humidity
  • Infant
  • Male
  • Middle Aged
  • Norovirus* / isolation & purification
  • Seasons*
  • Temperature
  • United States / epidemiology
  • Young Adult