Abstract
The effect of cobalt protoporphyrin IX (Co-haem) given to male rats in single subcutaneous doses (25-100 mumol/kg body wt.) was studied. Co-haem decreased cytochrome P-450 content and aminopyrine N-demethylase activity, but increased progesterone content and 3H-progesterone binding in a dose-related manner. The effect of a single dose of 50 mumol/kg body wt. was reversible; cytochrome P-450 and progesterone content, and progesterone binding, returned to the normal level 24-40 d after injection but aminopyrine N-demethylase activity was only partially restored. The converse actions of Co-haem on microsomal progesterone and cytochrome P-450 content showed high correlation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aminopyrine N-Demethylase / antagonists & inhibitors
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Aminopyrine N-Demethylase / metabolism
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Animals
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Cytochrome P-450 Enzyme Inhibitors
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Cytochrome P-450 Enzyme System / metabolism*
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Enzyme Induction / drug effects
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Heme / metabolism
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Injections, Subcutaneous
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Male
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Microsomes, Liver / drug effects
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Microsomes, Liver / metabolism*
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Porphyrins / pharmacology*
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Progesterone / metabolism*
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Protoporphyrins / administration & dosage
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Protoporphyrins / pharmacology*
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Rats
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Rats, Inbred F344
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Substrate Specificity
Substances
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Cytochrome P-450 Enzyme Inhibitors
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Porphyrins
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Protoporphyrins
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Heme
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Progesterone
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cobaltiprotoporphyrin
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Cytochrome P-450 Enzyme System
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Aminopyrine N-Demethylase