Association between progesterone binding and cytochrome P-450 content of hepatic microsomes in the rat treated with cobalt-haem

Xenobiotica. 1985 May;15(5):407-12. doi: 10.3109/00498258509045011.

Abstract

The effect of cobalt protoporphyrin IX (Co-haem) given to male rats in single subcutaneous doses (25-100 mumol/kg body wt.) was studied. Co-haem decreased cytochrome P-450 content and aminopyrine N-demethylase activity, but increased progesterone content and 3H-progesterone binding in a dose-related manner. The effect of a single dose of 50 mumol/kg body wt. was reversible; cytochrome P-450 and progesterone content, and progesterone binding, returned to the normal level 24-40 d after injection but aminopyrine N-demethylase activity was only partially restored. The converse actions of Co-haem on microsomal progesterone and cytochrome P-450 content showed high correlation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminopyrine N-Demethylase / antagonists & inhibitors
  • Aminopyrine N-Demethylase / metabolism
  • Animals
  • Cytochrome P-450 Enzyme Inhibitors
  • Cytochrome P-450 Enzyme System / metabolism*
  • Enzyme Induction / drug effects
  • Heme / metabolism
  • Injections, Subcutaneous
  • Male
  • Microsomes, Liver / drug effects
  • Microsomes, Liver / metabolism*
  • Porphyrins / pharmacology*
  • Progesterone / metabolism*
  • Protoporphyrins / administration & dosage
  • Protoporphyrins / pharmacology*
  • Rats
  • Rats, Inbred F344
  • Substrate Specificity

Substances

  • Cytochrome P-450 Enzyme Inhibitors
  • Porphyrins
  • Protoporphyrins
  • Heme
  • Progesterone
  • cobaltiprotoporphyrin
  • Cytochrome P-450 Enzyme System
  • Aminopyrine N-Demethylase